KLH Rabbit Polyclonal Antibody

CAT#: AP09215TR-N

KLH rabbit polyclonal antibody, Texas Red

Specifications

Product Data

• Applications: FC, IF
• Recommended Dilution: Suitable for most immunological techniques requiring high titer binding and lot-to-lot consistency.
  Flow cytometry: 1:2,000 - 1:10,000.
  Immunoflourescence: 1:500 - 1:2,500.
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: KLH (Keyhole Limpet Hemocyanin
• Specificity: This antibody detects KLH.
• Formulation: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/ml Bovine Serum Albumin (BSA) IgG and Protease free, 0.01% (w/v) Sodium Azide
  Label: Texas Red
  State: Lyophilized Ig fraction
  Label: (TM) Sulfonyl Chloride (Molecular Weight 625 daltons)
  Absorption emission: 596 nm / 620 nm
  Molar radio: 3.8 moles Texas RedTM per mole of Rabbit IgG
• Reconstitution Method: Restore with 0.1 ml of deionized water (or equivalent).
• Concentration: 1.0 mg/ml (by UV absorbance at 280 nm)
• Purification: Prepared from monospecific antiserum by immunoaffinity chromatography using KLH coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum.
• Conjugation: Texas Red
• Background: KLH (Keyhole Limpet Hemocyanin) is a copper containing oxygen carrier occurring freely dissolved in the hemolymph of many molluscs and arthropods. KLH is often used in molecular immunology as a carrier protein conjugated to low molecular weight molecules such as peptides, amino acids, nucleic acids, drugs or toxins to render them more immunogenic due to the size of the conjugate complex and the immunogenicity of KLH. KLH forms a large complex composed of ~50 kDa subunits.
• Synonyms: Keyhole Limpet Hemocyanine