Transferrin (TF) Rabbit Polyclonal Antibody

CAT#: AP09216HR-N

Transferrin (TF) rabbit polyclonal antibody, HRP

Specifications

Product Data

• Applications: ELISA, IHC, WB
• Recommended Dilution: Suitable for immunoblotting (western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
  ELISA: 1:3,000 - 1:12,000.
  Western blot: 1:300 - 1:1,000.
• Reactivities: Human
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Human Transferrin
• Specificity: This antibody is specific for Transferrin (TF).
• Formulation: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/ml Bovine Serum Albumin (BSA) IgG and Protease free, 0.01% (w/v) Gentamicin Sulfate
  Label: HRP
  State: Lyophilized Ig fraction
  Label: Horseradish Peroxidase
• Reconstitution Method: Restore with 0.5 ml of deionized water (or equivalent).
• Concentration: lot specific
• Purification: This reagent was purified from pooled rabbit sera obtained from rabbits immunized with Human Transferrin. Purification was by Protein A Sepharose Fast Flow followed by POROS EP antigen resin affinity purification. The purified antibody was then conjugated to horseradish peroxidase and subsequently purified followed by exhaustive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, purified Human Transferrin and Human Serum.
• Conjugation: HRP
• Storage: Store vial at 2-8 ° C prior to restoration. Following restoration product can be stored undiluted at 2-8 ° for up to one month or (in aliquots) at -20 °C or below. For extended storage add glycerol to 50%.
  Avoid repeated freezing and thawing. Centrifuge product if not completely clear after standing at room
  temperature.
• Stability: Shelf life: One year from despatch.
• Database Link:
  Entrez Gene 7018 Human
• Synonyms: Serotransferrin, Siderophilin