Transferrin (TF) Rabbit Polyclonal Antibody

CAT#: AP09216HR-N

Transferrin (TF) rabbit polyclonal antibody, HRP


USD 610.00

3 Days*

Size
    • 500 ug

Product Images

Specifications

Product Data
Applications ELISA, IHC, WB
Recommended Dilution Suitable for immunoblotting (western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
ELISA: 1:3,000 - 1:12,000.
Western blot: 1:300 - 1:1,000.
Reactivities Human
Host Rabbit
Isotype IgG
Clonality Polyclonal
Immunogen Human Transferrin
Specificity This antibody is specific for Transferrin (TF).
Formulation 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/ml Bovine Serum Albumin (BSA) IgG and Protease free, 0.01% (w/v) Gentamicin Sulfate
Label: HRP
State: Lyophilized Ig fraction
Label: Horseradish Peroxidase
Reconstitution Method Restore with 0.5 ml of deionized water (or equivalent).
Concentration lot specific
Purification This reagent was purified from pooled rabbit sera obtained from rabbits immunized with Human Transferrin. Purification was by Protein A Sepharose Fast Flow followed by POROS EP antigen resin affinity purification. The purified antibody was then conjugated to horseradish peroxidase and subsequently purified followed by exhaustive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, purified Human Transferrin and Human Serum.
Conjugation HRP
Storage Store vial at 2-8 ° C prior to restoration. Following restoration product can be stored undiluted at 2-8 ° for up to one month or (in aliquots) at -20 °C or below. For extended storage add glycerol to 50%.
Avoid repeated freezing and thawing. Centrifuge product if not completely clear after standing at room
temperature.
Stability Shelf life: One year from despatch.
Synonyms Serotransferrin, Siderophilin
Reference Data

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.