GFP (Ads. to Hu, Ms, Rt Serum Proteins) Rabbit Polyclonal Antibody

CAT#: AP09218PU-N

GFP (Ads. to Hu, Ms, Rt Serum Proteins) rabbit polyclonal antibody, Aff - Purified


USD 400.00

2 Weeks*

Size
    • 100 ug

Product Images

Specifications

Product Data
Applications ELISA, IF, IHC, WB
Recommended Dilution ELISA: 1/20,000-1/120,000.
IF Microscopy: 1/500-1/5,000.
Western Blot: 1/500-1/5,000.
Immunohistochemistry: 1/200-1/3,000.
Reactivities A. victoria
Host Rabbit
Isotype IgG
Clonality Polyclonal
Immunogen Green Fluorescent Protein (GFP) fusion protein corresponding to the full length amino acid sequence (246aa) derived from the jellyfish Aequorea victoria
Specificity Polyclonal anti-GFP is designed to detect GFP and its variants such as rGFP, eGFP, S65T-GFP, RS-GFP, YFP and EGF. 
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum and purified and partially purified Green Fluorescent Protein (Aequorea victoria). No reaction was observed against Human, Mouse or Rat serum proteins.
Formulation 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
State: Aff - Purified
State: Liquid sterile filtered Ig fraction
Stabilizer: None
Preservative: 0.01% (w/v) Sodium Azide
Concentration 1.0 mg/ml (by UV absorbance at 280 nm)
Purification This product was prepared from monospecific antiserum by immunoaffinity chromatography using Green Fluorescent Protein (Aequorea victoria) coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities
Predicted Protein Size 27 kDa
Background Green fluorescence protein (GFP) is a 27 kDa protein derived from the jellyfish Aequorea victoria, which emits green light (emission peak at a wavelenth of 509 nm) when excited by blue light (excitation peak at a wavelenth of 395 nm). Green Fluorescent Protein (GFP) has become an invaluable tool in cell biology research, since its intrinsic fluorescence can be visualized in living cells. GFP fluorescence is stable under fixation conditions and suitable for a variety of applications. GFP has been widely used as a reporter for gene expression, enabling researchers to visualize and localize GFP-tagged proteins within living cells without the need for chemical staining. Other applications of GFP include assessment of protein protein interactions through the yeast two hybrid system and measurement of distance between proteins through fluorescence energy transfer (FRET) protocols. GFP technnology has considerably contributed to a greater understanding of cellular physiology.
Synonyms Green fluorescent protein, GFP-Tag
Reference Data

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.