lacI Rabbit Polyclonal Antibody

CAT#: AP09404PU-S

lacI rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot using Affinity purified anti-Lac I antibody shows detection of a 38 kDa band corresponding to recombinant Lac I (arrowhead). The blot was blocked with 5% BLOTTO in TTBS overnight at 4°C. Primary antibody was used at a 1/1,000 dilution followed by reaction with a 1/10,000 dilution of HRP Goat anti-Rabbit IgG. ECL was used for detection.

Specifications

Product Data

• Applications: ELISA, WB
• Recommended Dilution: ELISA: 1/90,000–1/100,000.
  Western Blot: 1/500-1/2,000.
  Expect a band approximately ~38 kDa in size corresponding to Lac I by Western blotting in the appropriate cell lysate or extract.
• Reactivities: Escherichia coli
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Full-length recombinant Lac I protein
• Specificity: This antibody reacts with recombinant Lac I protein.
  A BLAST analysis was used to suggest cross-reactivity with Lac I from DNA-binding transcriptional repressor in Escherichia coli (strain DH10B) and transcriptional repressor of the lac operon in Shigella sonnei (strain Ss046) based on a 100% homology with the immunizing sequence.
• Formulation: 0.02M Potassium Phosphate, 0.15M Sodium Chloride, pH 7.2 containing 0.01% (w/v) Sodium Azide as preservative
  State: Aff - Purified
  State: Liquid (sterile filtred) purified Ig
• Concentration: 1.0 mg/ml (by UV absorbance at 280 nm)
• Purification: Affinity Chromatography
• Background: The lac repressor protein (Lac I) is an important genetic control protein. It controls the expression of the lactose metabolizing enzymes in Escherichia coli. This regulation involves specific recognition of the operator DNA sequence (Lac O) and consequent inhibition of transcription initiation by RNA polymerase; modulation of binding at this target site by small sugar molecules, inducers, provides the basis for regulation. Different ligand binding activities are associated with the isolated domains of Lac I: specific and nonspecific DNA binding with the NH2 termini, and inducer and specific DNA binding with the core protein. This antibody could possibly be used to recover and purify protein/nucleic acid complexes (host and viral proteins associated with the viral DNA) from infected cells.
• Synonyms: Lactose operon repressor, b0345, JW0336