FITC F(ab)2 Fragment Goat Polyclonal Antibody

CAT#: AP09428AP-N

FITC F(ab)2 Fragment goat polyclonal antibody, AP

Specifications

Product Data

• Applications: ELISA, IHC, WB
• Recommended Dilution: ELISA: 1:2,000 - 1:12,000.
  Western blot: 1:500 - 1:2,500.
  Dot blot.
  Immunohistochemistry on frozen sections: 1:200 - 1:1,000.
• Host: Goat
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Fluorescein conjugated to Goat IgG
• Specificity: This antibody detects Fluorescein F(ab')2.
  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase, anti-Goat Serum and Fluorescein conjugated Bovine Serum Albumin. No reaction was observed against anti-Pepsin or anti-Goat IgG F(c).
• Formulation: 0.05M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0; 10 mg/ml Bovine Serum Albumin (BSA), IgG and Protease free; 0.01% (w/v) Sodium Azide
  Label: AP
  State: Liquid sterile filtered Ig fraction
  Label: Alkaline Phosphatase (Calf Intestine); Molecular Weight 140,000 daltons
• Concentration: 1.0 mg/ml (by UV absorbance at 280 nm)
• Purification: Prepared from monospecific antiserum by immunoaffinity chromatography using Fluorescein IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.
• Conjugation: AP
• Synonyms: Fluorescein Isothiocyanate