FITC F(ab)2 Fragment Goat Polyclonal Antibody

CAT#: AP09428BT-N

FITC F(ab)2 Fragment goat polyclonal antibody, Biotin

Specifications

Product Data

• Applications: ELISA, IHC, WB
• Recommended Dilution: ELISA:1:20,000 - 1:100,000.
  Western blot: 1:2,000 - 1:10,000.
  Immunohistochemistry: 1:1,000 - 1:5,000.
• Host: Goat
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Fluorescein conjugated to Goat IgG
• Specificity: This antibody detetcs Fluorescein F(ab')2.
  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Goat Serum and Fluorescein conjugated BSA. No reaction was observed against anti-Pepsin or anti-Goat IgG F(c).
• Formulation: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2; 10 mg/ml Bovine Serum Albumin (BSA) IgG and Protease free; 0.01% (w/v) Sodium Azide
  Label: Biotin
  State: Lyophilized Ig fraction
  Label: Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC)
  Molar radio: 10-20 BAC molecules per Goat F(ab')2 molecule
• Reconstitution Method: Restore with 1.0 ml of deionized water (or equivalent).
• Concentration: 1.0 mg/ml (by UV absorbance at 280 nm)
• Purification: Immunoaffinity chromatography using Fluorescein coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation
• Conjugation: Biotin
• Synonyms: Fluorescein Isothiocyanate