FITC F(ab)2 Fragment Goat Polyclonal Antibody
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Specifications
Product Data | |
Applications | FC, IF |
Recommended Dilution | Suitable for highly specific immunological methods requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity. Flow cytometry: 1:2,000 - 1:10,000. Immunoflourescence: 1:500 - 1:2,500. |
Host | Goat |
Isotype | IgG |
Clonality | Polyclonal |
Immunogen | Fluorescein conjugated to Goat IgG |
Specificity | This antibody detects Fluorescein F(ab')2. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum and Fluorescein conjugated BSA. No reaction was observed against anti-Pepsin or anti-Goat IgG F(c). |
Formulation | 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2; 10 mg/ml Bovine Serum Albumin (BSA) IgG and Protease free; 0.01% (w/v) Sodium Azide Label: TRITC State: Lyophilized Ig fraction Label: Tetramethylrhodamine isothiocyanante (Molecular Weight 444 daltons) Absorption emission: 550 nm / 570 nm Molar radio: 2.0 moles TRITC per mole of Goat IgG |
Reconstitution Method | Restore with 1.0 ml of deionized water (or equivalent). |
Concentration | 1.0 mg/ml (by UV absorbance at 280 nm) |
Purification | Prepared from monospecific antiserum by immunoaffinity chromatography using Fluorescein coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation |
Conjugation | TRITC |
Synonyms | Fluorescein Isothiocyanate |
Reference Data |
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