FITC F(ab)2 Fragment Goat Polyclonal Antibody

CAT#: AP09428TC-N

FITC F(ab)2 Fragment goat polyclonal antibody, TRITC

Specifications

Product Data

• Applications: FC, IF
• Recommended Dilution: Suitable for highly specific immunological methods requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
  Flow cytometry: 1:2,000 - 1:10,000.
  Immunoflourescence: 1:500 - 1:2,500.
• Host: Goat
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Fluorescein conjugated to Goat IgG
• Specificity: This antibody detects Fluorescein F(ab')2.
  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum and Fluorescein conjugated BSA. No reaction was observed against anti-Pepsin or anti-Goat IgG F(c).
• Formulation: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2; 10 mg/ml Bovine Serum Albumin (BSA) IgG and Protease free; 0.01% (w/v) Sodium Azide
  Label: TRITC
  State: Lyophilized Ig fraction
  Label: Tetramethylrhodamine isothiocyanante (Molecular Weight 444 daltons)
  Absorption emission: 550 nm / 570 nm
  Molar radio: 2.0 moles TRITC per mole of Goat IgG
• Reconstitution Method: Restore with 1.0 ml of deionized water (or equivalent).
• Concentration: 1.0 mg/ml (by UV absorbance at 280 nm)
• Purification: Prepared from monospecific antiserum by immunoaffinity chromatography using Fluorescein coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation
• Conjugation: TRITC
• Synonyms: Fluorescein Isothiocyanate