Biotin (F(ab')2 fragment) Goat Polyclonal Antibody

CAT#: AP09429HR-N

Biotin (F(ab')2 fragment) goat polyclonal antibody, HRP

Specifications

Product Data

• Applications: ELISA, IHC, WB
• Recommended Dilution: Western Blot: 1/200 - 1/1,000.
  Dot Blot.
  ELISA: 1/2,000 - 1/10,000.
  Immunohistochemistry: 1/100 - 1/500.
• Host: Goat
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Biotin conjugated to Keyhole Limpet Hemocyanin (KLH)
• Specificity: This is a Peroxidase conjugated F(ab)2 fragment of Anti-Biotin.
• Formulation: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 10 mg/ml Bovine Serum Albumin (BSA) and 0.01% (w/v) Sodium Azide
  Label: HRP
  State: Lyophilized F(ab)2 fragment
  Label: Horseradish Peroxidase (Molecular Weight 40,000 daltons)
• Reconstitution Method: Restore with 1.0 ml of deionized water (or equivalent).
  For extended storage reconstitute product with 50% glycerol instead of water
• Concentration: 1,0 mg/ml (by UV absorbance at 280 nm)
• Purification: Immunoaffinity chromatography
• Conjugation: HRP
• Background: Biotin is a water soluble vitamin, generally classified as a B complex vitamin, also called vitamin B4. After the initial discovery of biotin, nearly forty years of research were required to establish it as a vitamin. Biotin is required by all organisms but can only be synthesized by bacteria, yeasts, molds, algae, and some plant species. Biotin is required as prosthetic group of enzymes involved in incorporation of carbon dioxide into organic compounds. Biotin has a MW of 244 Da.
• Synonyms: Vitamin B7, Vitamin H