Biotin (F(ab')2 fragment) Goat Polyclonal Antibody

CAT#: AP09429RP-N

Biotin (F(ab')2 fragment) goat polyclonal antibody, PE

Specifications

Product Data

• Applications: ELISA, FC, IF, WB
• Recommended Dilution: Immunofluorescence.
  Flow Cytometry: 1/100 - 1/250.
  Western Blot: 1/100-1/250.
  ELISA: 1/100-1/250.
• Host: Goat
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Biotin conjugated to Keyhole Limpet Hemocyanin (KLH)
• Specificity: This is a Phycoerythrin Conjugated F(ab)2 fragment of Anti-Biotin.
• Formulation: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 10 mg/ml Bovine Serum Albumin (BSA) and 0.01% (w/v) Sodium Azide
  Label: PE
  State: Liquid F(ab)2 fragment
  Label: R-Phycoerythrin ( Molecular Weight 240,000 daltons)
  Absorption emission: 488 nm / 575 nm
• Reconstitution Method: Restore with 1.0 ml of deionized water (or equivalent).
• Concentration: 0.5 mg/ml (by absorbance = 82.0 at 565 nm)
• Purification: Immunoaffinity chromatography
• Conjugation: PE
• Background: Biotin is a water soluble vitamin, generally classified as a B complex vitamin, also called vitamin B4. After the initial discovery of biotin, nearly forty years of research were required to establish it as a vitamin. Biotin is required by all organisms but can only be synthesized by bacteria, yeasts, molds, algae, and some plant species. Biotin is required as prosthetic group of enzymes involved in incorporation of carbon dioxide into organic compounds. Biotin has a MW of 244 Da.
• Synonyms: Vitamin B7, Vitamin H