Biotin (F(ab')2 fragment) Goat Polyclonal Antibody

CAT#: AP09429TR-N

Biotin (F(ab')2 fragment) goat polyclonal antibody, Texas Red

Specifications

Product Data

• Applications: FC, IF
• Recommended Dilution: Immunofluorescence.
  Flow Cytometry.
• Host: Goat
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Biotin conjugated to Keyhole Limpet Hemocyanin (KLH)
• Specificity: This is a Texas Red(TM) Conjugated F(ab)2 fragment of Anti-Biotin.
• Formulation: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 10 mg/ml Bovine Serum Albumin (BSA) and 0.01% (w/v) Sodium Azide
  Label: Texas Red
  State: Lyophilized F(ab)2 fragment
  Label: (TM) Sulfonyl Chloride (Molecular Weight 625 daltons)
  Absorption emission: 596 nm / 620 nm
  Molar radio: 4.0 moles Texas Red(TM) per mole of Goat IgG F(ab)2
• Reconstitution Method: Restore with 1.0 ml of deionized water (or equivalent).
  For extended storage reconstitute product with 50% glycerol instead of water
• Concentration: 1,0 mg/ml (by UV absorbance at 280 nm)
• Purification: Immunoaffinity chromatography
• Conjugation: Texas Red
• Background: Biotin is a water soluble vitamin, generally classified as a B complex vitamin, also called vitamin B4. After the initial discovery of biotin, nearly forty years of research were required to establish it as a vitamin. Biotin is required by all organisms but can only be synthesized by bacteria, yeasts, molds, algae, and some plant species. Biotin is required as prosthetic group of enzymes involved in incorporation of carbon dioxide into organic compounds. Biotin has a MW of 244 Da.
• Synonyms: Vitamin B7, Vitamin H