alpha Synuclein (SNCA) pTyr133 Rabbit Polyclonal Antibody

CAT#: AP09477PU-N

alpha Synuclein (SNCA) pTyr133 rabbit polyclonal antibody, Aff - Purified

Product Images

Western Blot analysis of extracts from C2C12 cells untreated or treated with Anisomycin using alpha-Synuclein antibody (pTyr133).

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  Immunofluorescence staining of methanol-fixed HeLa cells using apha-Synuclein antibody (pTyr133).

Specifications

Product Data

• Applications: IF, WB
• Recommended Dilution: Western Blot: 1/500-1/1000.
  Immunofluorescence: 1/100-1/200.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Peptide Sequence around the phosphorylation site of Tyr133 (E-G-Yp-Q-D) derived from Human Alpha-Synuclein
• Specificity: This antibody detects endogenous levels of Alpha-Synuclein only when phosphorylated at Tyrosine 133.
• Formulation: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02% Sodium Azide and 50% Glycerol.
  State: Aff - Purified
  State: Liquid purified Ig fraction.
• Concentration: lot specific
• Purification: Immunoaffinity Chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
• Storage: Store the antibody (in aliquots) at -20°C.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: One year from despatch.
• Gene Name: Homo sapiens synuclein, alpha (non A4 component of amyloid precursor) (SNCA), transcript variant 1
• Database Link:
  Entrez Gene 20617 MouseEntrez Gene 29219 RatEntrez Gene 6622 Human
• Background: Alpha Synuclein is implicated in the regulation of dopamine release and transport. It is a soluble protein, expressed principally in the brain but also expressed in low concentrations in all tissues examined (except liver). In the nervous system, alpha Synuclein is primarily located at presynaptic terminals and is found membrane bound in dopaminergic neurons. It can form filamentous aggregates that are the major non amyloid component of intracellular inclusions in several neurodegenerative diseases (synucleinopathies), including Parkinson's Disease. Alpha Synuclein induces fibrillization of microtubule associated protein tau and reduces neuronal responsiveness to various apoptotic stimuli, leading to a decreased caspase 3 activation. Alpha synuclein is a protein phosphorylated predominantly on serine residues.
• Synonyms: NACP, PARK1
• Note: Molecular Weight: 18 kDa

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Alzheimer's disease, Parkinson's disease