MARCKS pSer162 Rabbit Polyclonal Antibody

CAT#: AP09479PU-S

MARCKS pSer162 rabbit polyclonal antibody, Aff - Purified

Product Images

Figure 1. Immunofluorescence staining of methanol-fixed HeLa cells using MARCKS pSer162 Antibody (Red).

Specifications

Product Data

• Applications: IF
• Recommended Dilution: Immunofluorescence: 1/100~1/200.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: The antiserum was produced against synthesized phosphopeptide derived from human MARCKS around the phosphorylation site of serine 162 (K-K-Sp-F-K).
• Specificity: Antibody AP09479PU detects endogenous levels of MARCKS only when phosphorylated at Serine 162.
• Formulation: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02% Sodium Azide and 50% Glycerol.
  State: Aff - Purified
  State: Liquid purified Ig fraction.
• Concentration: lot specific
• Purification: Immunoaffinity Chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
• Storage: Store the antibody (in aliquots) at -20°C.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: One year from despatch.
• Gene Name: Homo sapiens myristoylated alanine rich protein kinase C substrate (MARCKS)
• Database Link:
  Entrez Gene 17118 MouseEntrez Gene 25603 RatEntrez Gene 4082 Human
• Background: MARCKS, (Myristoylated Alanine-Rich C Kinase Substrate), is a member of a family of calmodulin binding proteins and is a major substrate for phosphorylation by protein kinase C (PKC). The phosphorylation of Ser152/156 can be used as a measure of PKC activation. Phosphorylation of Ser152/156 modulates the binding of MARCKS to calmodulin.
• Synonyms: Myristoylated alanine-rich C-kinase substrate, MACS, PRKCSL

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Fc gamma R-mediated phagocytosis