DARPP32 (PPP1R1B) pThr75 Rabbit Polyclonal Antibody

CAT#: AP09490PU-S

DARPP32 (PPP1R1B) pThr75 rabbit polyclonal antibody, Aff - Purified

Product Images

Immunofluorescence staining of methanol-fixed HeLa cells using DARPP-32 (Phospho-Thr75) Antibody

Specifications

Product Data

• Applications: IF
• Recommended Dilution: Immunofluorescence: 1/100 - 1/200.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Synthesized KLH phosphopeptide derived from human DARPP-32 around the phosphorylation site of threonine 75 (A-Y-TP-P-P).
  
  
• Specificity: DARPP-32 (Phospho-Thr75) Antibody detects endogenous levels of DARPP-32 only when phosphorylated at threonine 75.
• Formulation: Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol
  State: Aff - Purified
  State: Liquid purified IgG
• Concentration: 1.0 mg/ml
• Purification: Affinity chromatography
• Predicted Protein Size: 32 kd.
• Gene Name: Homo sapiens protein phosphatase 1 regulatory inhibitor subunit 1B (PPP1R1B), transcript variant 1
• Database Link:
  Entrez Gene 19049 MouseEntrez Gene 360616 RatEntrez Gene 84152 Human
• Background: DARPP32 is expressed in medium-sized spiny neurons that also express dopamine D1 receptors. The function of DARPP32 seems to be regulated by receptor stimulation. Both dopaminergic and glutamatergic (NMDA) receptor stimulation regulate the extent of DARPP32 phosphorylation, but in opposite directions. Dopamine D1 receptor stimulation enhances cAMP formation, resulting in the phosphorylation of DARPP32 and phosphorylated DARPP32 is a potent protein phosphatase 1. NMDA receptor stimulation elevates intracellular calcium, which leads to activation of calcineurin and dephosphorylation of phospho DARPP32, thereby reducing the phosphatase 1 inhibitory activity of DARPP32.
• Synonyms: PPP1R1B, DARPP-32, FLJ20940

Reference Data

• Protein Families: Druggable Genome