eNOS (NOS3) Rabbit Polyclonal Antibody

CAT#: AP09503PU-S

eNOS (NOS3) rabbit polyclonal antibody, Aff - Purified

Product Images

Immunofluorescence staining of methanol-fixed HeLa cells using eNOS Antibody

Specifications

Product Data

• Applications: IF
• Recommended Dilution: Immunofluorescence: 1/100 - 1/200.
• Reactivities: Human Mouse Rat
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Synthesized non-phosphopeptide derived from human eNOS around the phosphorylation site of threonine 495(K-K-TP-F-K)
• Specificity: eNOS Antibody detects endogenous levels of total eNOS protein.
• Formulation: Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol
  State: Aff - Purified
  State: Liquid purified Ig
• Concentration: lot specific
• Purification: Affinity chromatography
• Storage: Store the antibody at -20°C.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: Homo sapiens nitric oxide synthase 3 (NOS3), transcript variant 1
• Database Link:
  Entrez Gene 18127 Mouse
• Background: Nitric oxide synthase NOS oxidizes a guanidine nitrogen of arginine releasing nitric oxide in the form of a free radical and citrulline. Nitric oxide thus generated acts as a messenger in diverse functions including vasodilation neurotransmission, anti tumor and anti pathogenic activities. NOS is classified under three types: neuronal NOS (nNOS) or brain NOS (bNOS); inducible NOS (iNOS) or macrophage NOS (mNOS); and endothelial NOS (eNOS).
  eNOS is a calcium/calmodulin dependent enzyme which undergoes several post translational modifications, including acylation with myristate and palmitate, and phosphorylation on numerous residues. As with the other members of the NOS family, eNOS derives the diffusible multifunctional second messenger NO from L arginine through a series of reactions in which L citrulline is an intermediate. eNOS plays an important role in controlling vascular tone, platelet aggregation, and cardiac myocyte function.
• Synonyms: Endothelial NOS, EC-NOS, NOS type III, Constitutive NOS, eNOS

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Alzheimer's disease, Amyotrophic lateral sclerosis (ALS), Arginine and proline metabolism, Calcium signaling pathway, Long-term depression, Metabolic pathways, Pathways in cancer, Small cell lung cancer, VEGF signaling pathway