Synaptotagmin 1 (SYT1) Rabbit Polyclonal Antibody

CAT#: AP09507PU-S

Synaptotagmin 1 (SYT1) rabbit polyclonal antibody, Aff - Purified

Product Images

Western Blot analysis of extracts from mouse brain and rat brain tissue using Synaptotagmin antibody

• 

  Immunofluorescence staining of methanol-fixed HeLa cells using Synaptotagmin Antibody

Specifications

Product Data

• Applications: IF, WB
• Recommended Dilution: Immunofluorescence: 1/100 - 1/200.
  Western Blot: 1/500 - 1/1000.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Synthesized non-phosphopeptide derived from human Synaptotagmin around the phosphorylation site of serine 309 (G-L-SP-D-P)
• Specificity: Synaptotagmin Antibody detects endogenous levels of total Synaptotagmin protein.
• Formulation: Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol
  State: Aff - Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Affinity chromatography
• Storage: Store the antibody at -20°C.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: Homo sapiens synaptotagmin 1 (SYT1), transcript variant 2
• Database Link:
  Entrez Gene 20979 MouseEntrez Gene 25716 RatEntrez Gene 6857 Human
• Background: Synaptotagmin 1 is a 60kDa member of the synaptotagmin family, expressed in membranes of synaptic vesicles in rostral, phylogenetically younger brain regions and in some endocrine tissues. Ca2+ is bound by synaptogamin whose C2 domains then bind phospholipids, trafficking vesicles to the synaptic membrane and exocytosis. This results in fast synchronous neurotransmitter release. Synaptotagmin also binds neurexins, syntaxin and AP2 independently of Ca2+ binding. Phosphorylation of synaptotagmin by protein kinases is thought to modulate its activity.
• Synonyms: SYT1, SVP65, SYT, Synaptotagmin1, Synaptotagmin I, SytI, p65

Reference Data

• Protein Families: Secreted Protein, Transmembrane