c Kit (KIT) Rabbit Polyclonal Antibody

CAT#: AP09513PU-N

c Kit (KIT) rabbit polyclonal antibody, Aff - Purified

Product Images

Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using c-kit Antibody

Specifications

Product Data

• Applications: IHC
• Recommended Dilution: Immunohistochemistry: 1/50-1/100.
• Reactivities: Human
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: peptide sequence around amino acids 934~938 (H-I-Y-S-N) derived from Human c-kit.
• Specificity: c-kit Antibody detects endogenous levels of total c-kit protein.
• Formulation: Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% Sodium Azide and 50% Glycerol
  State: Aff - Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Affinity Chromatography using epitope-specific peptide
• Storage: Store the antibody at -20°C.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: Homo sapiens KIT proto-oncogene receptor tyrosine kinase (KIT), transcript variant 1
• Database Link:
  Entrez Gene 3815 Human
• Background: c-Kit is a transmembrane tyrosine kinase encoded by the cKit proto oncogene. c-Kit acts to regulate a variety of biological responses including cell proliferation, apoptosis, chemotaxis and adhesion. Ligand binding to the extracellular domain leads to autophosphorylation on several tyrosine residues within the cytoplasmic domain, and activation. Mutations in c-Kit have been found to be important for tumor growth and progression in a variety of cancers including mast cell diseases, gastrointestinal stromal tumor, acute myeloid leukemia, Ewing sarcoma and lung cancer. Phosphorylation at tyrosine 721 of c-Kit allows binding and activation of PI3 kinase.
• Synonyms: SCFR, KIT
• Note: Molecular Weight: 145 kDa

Reference Data

• Protein Families: Adult stem cells, Druggable Genome, Embryonic stem cells, ES Cell Differentiation/IPS, Protein Kinase, Stem cell - Pluripotency, Transmembrane
• Protein Pathways: Acute myeloid leukemia, Cytokine-cytokine receptor interaction, Endocytosis, Hematopoietic cell lineage, Melanogenesis, Pathways in cancer