FAK (PTK2) Rabbit Polyclonal Antibody

CAT#: AP09515PU-N

FAK (PTK2) rabbit polyclonal antibody, Aff - Purified

Product Images

Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using FAK Antibody

Specifications

Product Data

• Applications: IHC
• Recommended Dilution: Immunohistochemistry: 1/50 - 1/100.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Synthesized non-phosphopeptide derived from human FAK around the phosphorylation site of tyrosine 576/577 (T-Y-YP-K-A)
• Specificity: FAK Antibody detects endogenous levels of total FAK protein.
• Formulation: Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol
  State: Aff - Purified
  State: Liquid purified Ig
• Concentration: lot specific
• Purification: Affinity chromatography
• Storage: Store the antibody at -20°C.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: Homo sapiens protein tyrosine kinase 2 (PTK2), transcript variant 2
• Database Link:
  Entrez Gene 14083 MouseEntrez Gene 25614 RatEntrez Gene 5747 Human
• Background: Focal adhesion kinase (FAK) is a non receptor protein tyrosine kinase discovered as a substrate for Src and as a key element of integrin signaling. FAK plays a central role in cell spreading, differentiation, migration, cell death and acceleration of the G1 to S phase transition of the cell cycle. FAK regulation includes phosphorylation at multiple tyrosine and serine residues. Phosphorylation of tyrosine generally is associated with positive regulation and growth promotion, however, dephosphorylation at these sites occurs as cells enter mitosis (M-Phase of the cell cycle). In contrast, serine phosphorylation either remains high or is increased as cells enter mitosis and may play a role in focal adhesion disassembly.
  FAK and its phosphorylation states have been implicated in cancer metastasis and tumor cell survival and adhesion-independent growth. Additionally, recent evidence indicates that elevation of FAK activity in human carcinoma cells is associated with increased invasive potential. A central role in tumor formation and progression suggests that FAK is an attractive target for therapeutic intervention.
• Synonyms: FAK, Focal adhesion kinase 1, FADK1, pp125FAK, Protein-tyrosine kinase 2

Reference Data

• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Axon guidance, Chemokine signaling pathway, ErbB signaling pathway, Focal adhesion, Leukocyte transendothelial migration, Pathways in cancer, Regulation of actin cytoskeleton, Small cell lung cancer, VEGF signaling pathway