MET Rabbit Polyclonal Antibody

CAT#: AP09516PU-S

MET rabbit polyclonal antibody, Aff - Purified

Product Images

Figure 1. Western blot analysis of extracts from A431 and HepG2 cells using Met antibody.

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  Figure 2. Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue using Met antibody (Left) and the same antibody preincubated with blocking peptide (Right).

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: Western Blot: 1/500-1/1000.
  Incubate membrane with diluted antibody in 5% nonfat milk, 1xTBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight.
  Immunohistochemistry on Paraffin Embedded Tissue: 1/50-1/100.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Peptide sequence around amino acids 1001~1005 (V-D-Y-R-A) derived from Human Met.
• Specificity: The antibody detects endogenous levels of total Met protein.
• Formulation: PBS (without Mg2+ and Ca2+), pH 7.4, containing 150 mM NaCl, 0.02% Sodium Azide and 50% glycerol
  State: Aff - Purified
  State: Liquid purified IgG fraction.
• Concentration: lot specific
• Purification: Affinity Chromatography using epitope-specific immunogen.
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: Homo sapiens MET proto-oncogene, receptor tyrosine kinase (MET), transcript variant 2
• Database Link:
  Entrez Gene 17295 MouseEntrez Gene 24553 RatEntrez Gene 4233 Human
• Background: The c-Met oncogene was originally isolated from chemical carcinogen-treated human osteogenic sarcoma cell line by transfection analysis in NIH/3T3 cells. The Met proto-oncogene product was identified as a trans-membrane receptor-like protein with tyrosine kinase activity that is expressed in many tissues. The c-Met gene product has been identified as the cell surface receptor for hepatocyte growth factor, a plasminogen-like protein thought to be a humoral mediator of liver regeneration.
• Synonyms: Hepatocyte growth factor receptor, MET, Scatter factor receptor, HGF/SF receptor, c-Met
• Note: Molecular Weight: 156 kDa

Reference Data

• Protein Families: Druggable Genome, Protein Kinase, Transmembrane
• Protein Pathways: Adherens junction, Axon guidance, Colorectal cancer, Cytokine-cytokine receptor interaction, Endocytosis, Epithelial cell signaling in Helicobacter pylori infection, Focal adhesion, Melanoma, Pathways in cancer, Renal cell carcinoma