AHNAK Guinea Pig Polyclonal Antibody

CAT#: AP09541SU-N

AHNAK guinea pig polyclonal antibody, Serum

Specifications

Product Data

• Applications: IF, IHC, IP, WB
• Recommended Dilution: Western Blot: 1/1,000-1/2,000 (ECL).
  Immunoprecipitation.
  Immunofluorescence.
  Immunohistochemistry on Frozen Sections: 1/50-1/100.
  Incubation Time: 1 h at RT.
• Reactivities: Bovine, Human
• Host: Guinea Pig
• Clonality: Polyclonal
• Immunogen: Synthetic peptides of Human Desmoyokin (D1a/b, D2a/b, D4a/b), coupled to KLH
• Specificity: This antibody is specific for Desmoyokin (AHNAK antigen), an approx.600 kD protein localized e.g. in interdesmosomal membrane parts of epithelia and cortical lens fiber cells, but also found in the cytoplasm and nuclei of epithelia.
• Formulation: State: Serum
  State: Liquid Stabilized Antiserum
  Preservative: 0.09% Sodium Azide
• Gene Name: Homo sapiens AHNAK nucleoprotein (AHNAK), transcript variant 1
• Database Link:
  Entrez Gene 79026 Human
• Background: AHNAK1 (Desmoyokin) is a large (700 kDa) scaffold protein that translocates to the plasma membrane after an increas of extracellular calcium level or upon proteinkinase C activation and regulates extracellular calcium influx mediated by L-type Ca2+ channels. AHNAK1 has been implicated in diverse signal transduction proceses affecting cell differentiation and proliferation. In response to calcium-dependent intercellular contacts AHNAK1 forms multimeric complexes in the plasma membrane, connected with actin and annexin 2/S100A10 assemblies and is thus involved in organization of the plasma membrane architecture. In epithelial cells, AHNAK1 is localized in cytoplasm or is membrane-associated, but in cells of nonepithelial origin AHNAK1 is predominantly nuclear; it has a weak DNA-binding activity and associates with the DNA-ligase IV-XRCC4 complex.
• Synonyms: Desmoyokin

Reference Data

• Protein Families: Protease