Plakophilin 3 (PKP3) (277-474) Guinea Pig Polyclonal Antibody

CAT#: AP09555SU-N

Plakophilin 3 (PKP3) (277-474) guinea pig polyclonal antibody, Serum

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: Immunohiostochemistry on frozen and paraffin sections: 1/100.
  Western Blot: 1/1000.
  Immunofluorescence.
  Positive tissues:
  - stratified squamous epithelia: palmar, epidermis, scalp skin
  - stratified epithelia: tongue, vagina, esophagus
  - transitional epithelia: bladder
  - non-stratified (single layered) epithelia: colon, duodenum, pancreas, several glands
  - non-epithelial: reticulum cells of lymph node follicle
  Negative tissues:
  - liver, heart, skeletal muscle, endothelia (vessels)
• Reactivities: Human
• Host: Guinea Pig
• Clonality: Polyclonal
• Immunogen: Human recombinant plakophilin 3 peptide (aa 277-474) purified from E. coli
• Specificity: PP3-1 antiserum staining for plakophilin 3 is seen in most
  desmosomes and partly dispersed in cytoplasm under
  various fixation conditions (FA, MeAc long, MeAc short):
• Formulation: State: Serum
  State: Liquid serum containing 0.09% sodium azide
• Gene Name: Homo sapiens plakophilin 3 (PKP3), transcript variant 1
• Database Link:
  Entrez Gene 11187 Human
• Background: Plakophilin 3 is an Armadillo-like protein present in nuclei and desmosomes of epithelial cells. The expression pattern of this protein seems to be largely restricted to epithelial cell types. Plakophilin-3 can be detected along cell borders in a punctuate staining pattern typical for desmosomal proteins. In addition to the desmosomal immunolocalisation, immunostaining was observed as bright nuclear speckles. Thus, like plakophilin-1 and-2, plakophilin-3 displays a dual intracellular localisation in the desmosomal plaque and in the cell nucleus, and therefore is probably involved in signal transduction pathways between the plasma membrane and the nucleus. The human protein has a predicted molecular mass of 87 kD.
• Synonyms: Plakophilin-3