PRDM1 (C-term) Goat Polyclonal Antibody

CAT#: AP10057PU-N

PRDM1 (C-term) goat polyclonal antibody, Aff - Purified

Product Images

Anti-PRDM1 (1ug/ml) staining of Human Brain (cerebellum) lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Specifications

Product Data

• Applications: ELISA, WB
• Recommended Dilution: Peptide ELISA: 1/2000 (Detection Limit).
  Western blot: 1-3 µg/ml. Approx 100kDa band observed in Human Brain (cerebellum) lysates (calculated MW of 91.8kDa according to NP_001189.2 and of 76.8 according to NP_878911.1).
• Reactivities: Bovine, Canine, Human, Mouse, Porcine, Rat
• Host: Goat
• Clonality: Polyclonal
• Immunogen: Peptide with sequence C-KVKQETVEPMDP, from the C-Terminus of the protein sequence according to NP_001189.2; NP_878911.1.
• Specificity: This antibody is expected to recognize both reported isoforms (NP_001189.2 and NP_878911.1).
• Formulation: Tris saline, pH~7.3
  State: Aff - Purified
  State: Liquid purified Ig fraction
  Stabilizer: 0.5% BSA
  Preservative: 0.02% Sodium Azide
• Concentration: lot specific
• Purification: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide.
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: Homo sapiens PR/SET domain 1 (PRDM1), transcript variant 1
• Database Link:
  Entrez Gene 12142 MouseEntrez Gene 309871 RatEntrez Gene 639 Human
• Background: PRDM1/Blimp1 is a repressor of beta-interferon gene expression. This action is exerted by binding to the PRDI (positive regulatory domain I element) of the beta-IFN gene promoter. The transcription of this gene is increased upon virus induction.
• Synonyms: BLIMP-1, PRDI-BF1, PRDI-binding factor 1

Reference Data

• Protein Families: Transcription Factors