TGFBI Goat Polyclonal Antibody

CAT#: AP10415PU-N

TGFBI goat polyclonal antibody, Aff - Purified


USD 375.00

5 Days*

Size
    • 100 ug

Product Images

Specifications

Product Data
Applications ELISA, IHC, WB
Recommended Dilution Peptide ELISA: Detection Limit Dilution: 1/32000.
Western Blot: 0.2-0.5 µg/ml. Approx 70kDa band observed in Human, Mouse and Rat Kidney lysates (calculated MW of 74.7kDa according to Human NP_000349.1).
Immunohistochemistry on Paraffin Sections: 4-6 µg/ml.
In paraffin embedded Kidney shows staining of intercalated cells of a collecting tubule.
Reactivities Canine, Human, Mouse, Rat
Host Goat
Clonality Polyclonal
Immunogen Peptide with sequence from the Internal region of the protein sequence according to NP_000349.1.
Specificity Recognizes TGFBI/BIGH3.
Formulation Tris saline, pH 7.3 containing 0.02% Sodium Azide as preservative and 0.5% BSA as stabilizer.
State: Aff - Purified
State: Liquid purified IgG fraction.
Concentration lot specific
Purification Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide.
Storage Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Stability Shelf life: one year from despatch.
Background TGFBI, also known as BIGH3 and βig-h3, is an extracellular matrix protein induced by transforming growth factor (TGF)-beta 1. TGFBI protein is involved in cell growth, cell differentiation, wound healing and cell adhesion. In addition, some missense mutations of TGFBI were identified in families affected with human autosomal dominant corneal dystrophies. BIGH3 gene encodes for a 683 amino-acid protein containing an RGD motif and four internal repeated domains which have highly conserved sequences founded in several species (Fasciclin domain).
Synonyms Beta ig-h3, RGD-CAP, Kerato-epithelin
Reference Data

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.