LIN28B Rabbit Polyclonal Antibody

CAT#: AP11496PU-N

LIN28B rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot analysis of anti-LIN28B antibody in K562 cell line lysates (35 ug/lane). LIN28B (arrow) was detected using the purified Pab (1:60 dilution).

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  Formalin-fixed and paraffin-embedded human testis tissue reacted with LIN28B antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

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  Formalin-fixed and paraffin-embedded human Spleen tissue reacted with LIN28B antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

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  Confocal immunofluorescent analysis of LIN28B antibody (Center) with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

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  Flow Cytometric analysis of HL-60 cells using LIN28B antibody (Center) (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

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Specifications

Product Data

• Applications: FC, IF, IHC, WB
• Recommended Dilution: Western blot: 1/1000.
  Flow Cytometry: 1/10-1/50.
  Immunofluorescence: 1/10-1/50.
  Immunohistochemistry on Paraffin Sections: 1/10-1/50.
• Reactivities: Human, Mouse
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: KLH conjugated synthetic peptide between 95-128 amino acids from the Central region of Human LIN28B.
• Specificity: This antibody detects LIN28B at center.
• Formulation: PBS
  State: Aff - Purified
  State: Liquid purified Ig fraction
  Preservative: 0.09% (W/V) Sodium Azide
• Concentration: lot specific
• Purification: Purified by Protein A column followed by peptide affinity purifcation.
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 26953 Da
• Gene Name: lin-28 homolog B
• Database Link:
  Entrez Gene 389421 Human
• Background: Lin-28 homolog B (LIN28B) is overexpressed in hepatocellular carcinoma. The heterochronic gene lin-28 is a key regulator of developmental timing in the nematode Caenorhabditis elegans. Similar with lin-28 proteins, LIN28B conserves a cold shock domain and a pair of CCHC zinc finger domains. Phylogenetic analysis suggests that they might arise as a result of duplication from an ancestral gene. Overexpression of LIN28B was noted in most HCC cell lines and clinical samples. A short LIN28B isoform was also identified in non-tumor liver tissue and fetal liver. Although predominantly localized in the cytoplasm, LIN28B protein shows cell cycle-dependent nuclear translocation in Huh7 cells. Induced expression of exogenous LIN28B in a tet-off cell line promoted cancer cell proliferation.
• Synonyms: LIN28B, CSDD2, Lin-28 homolog B