IDO1 (C-term) Rabbit Polyclonal Antibody

CAT#: AP12352PU-N

IDO1 (C-term) rabbit polyclonal antibody, Purified

Product Images

Western blot analysis of anti-INDO Antibody (C-term) in 293 cell line lysates (35ug/lane). INDO (arrow) was detected using the purified Pab (1:60 dilution).

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  INDO antibody (C-term) staining of Formalin-Fixed and Paraffin-Embedded Human lung carcinoma tissue. The primary antibody was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: Peptide ELISA: 1/1,000.
  Western Blot: 1/50-1/100.
  Immunohistchemistry: 1/10-1/50.
• Reactivities: Human
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: KLH conjugated synthetic peptide between 374-403 amino acids from the C-terminal region of human INDO.
• Specificity: This antibody detects INDO (C-term).
• Formulation: PBS with 0.09% (W/V) Sodium Azide as preservative.
  State: Purified
  State: Liquid purified Ig fraction.
• Concentration: lot specific
• Purification: Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: indoleamine 2,3-dioxygenase 1
• Database Link:
  Entrez Gene 3620 Human
  UniProt ID P14902
• Background: Gamma-interferon (IFNG; MIM 147570) has an antiproliferative effect on many tumor cells and inhibits intracellular pathogens such as Toxoplasma and Chlamydia, at least partly because of the induction of indoleamine 2,3-dioxygenase (INDO; EC 1.13.11.52). This enzyme catalyzes the degradation of the essential amino acid L-tryptophan to N-formyl-kynurenine.
• Synonyms: IDO1, IDO, INDO, Indoleamine 2,3-dioxygenase 1, EC=1.13.11.52, Indoleamine 2,3-dioxygenase, IDO, Indoleamine-pyrrole 2,3-dioxygenase
• Note: Calculated Molecular weight: 45326 Da