Gremlin 1 (GREM1) (C-term) Rabbit Polyclonal Antibody

CAT#: AP13096PU-N

Gremlin 1 (GREM1) (C-term) rabbit polyclonal antibody, Purified

Product Images

(LEFT)Western blot analysis of anti-Gremlin Pab in CEM cell line lysates (35ug/lane). Gremlin (arrow) was detected using the purified Pab. (RIGHT)Western blot analysis of anti-Gremlin Pab in mouse liver cell lysate. Gremlin (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.

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  (TOP) Formalin-PBS and Bouin embedded paraffin human renal tissue pretreated with heat induced epitope retrieval Citrate buffer pH 6.0 in microwave. Dilution of primary antibody was 1:50. Detection method was ABC system or Vectastaint Elite VECTOR. (BUTTOM) Formalin-fixed and paraffin-embedded human skeletal muscle tissue reacted with GREMLIN Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: ELISA: 1/1,000.
  Western blotting: 1/100 - 1/500.
  Immunohistochemistry (Application validated by third-party citation).
• Reactivities: Human, Mouse
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminus of human Gremlin.
• Specificity: This antibody reacts to GREMLIN.
• Formulation: PBS with 0.09% (W/V) Sodium Azide as preservative
  State: Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS
• Storage:

  Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Gene Name: Homo sapiens gremlin 1, DAN family BMP antagonist (GREM1), transcript variant 1
• Database Link:
  Entrez Gene 23892 MouseEntrez Gene 26585 Human
• Background: GREMLIN is a member of the BMP (bone morphogenic protein) antagonist family. Like BMPs, BMP antagonists contain cystine knots and typically form homo- and heterodimers. The CAN (cerberus and dan) subfamily of BMP antagonists, to which this gene belongs, is characterized by a C-terminal cystine knot with an eight-membered ring. The antagonistic effect of the secreted glycosylated protein encoded by this gene is likely due to its direct binding to BMP proteins. As an antagonist of BMP, this gene may play a role in regulating organogenesis, body patterning, and tissue differentiation. In mouse, this protein has been shown to relay the sonic hedgehog (SHH) signal from the polarizing region to the apical ectodermal ridge during limb bud outgrowth.
• Synonyms: DAND2, CKTSF1B1, DRM, PIG2, IHG-2, GREM-1

Reference Data

• Protein Families: ES Cell Differentiation/IPS, Secreted Protein