Sclerostin (SOST) (N-term) Rabbit Polyclonal Antibody

CAT#: AP13235PU-N

Sclerostin (SOST) (N-term) rabbit polyclonal antibody, Purified

Product Images

Western blot analysis of anti-SOST Antibody (N-term) Pab in mouse liver tissue lysates (35ug/lane). SOST (arrow) was detected using the purified Pab (1:60 dilution).

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  Western blot analysis of lysates from human lung, mouse lung tissue lysate (from left to right), using SOST Antibody (N-term) ( ). was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20ug per lane.

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  Western blot analysis of lysates from Hela cell line, human lung, mouse lung, mouse liver tissue lysate (from left to right), using SOST Antibody (N-term). was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20ug per lane.

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  Western blot analysis of lysates from mouse liver, mouse lung, rat kidney tissue lysate (from left to right), using SOST Antibody (N-term). was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20ug per lane.

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  Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with SOST antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

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Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: ELISA: 1/1,000.
  Western blotting: 1/1,000.
  Immunohistochemistry: 1/10-1/50.
• Reactivities: Human, Mouse
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: KLH conjugated synthetic peptide between 12-42 amino acids from the N-terminal region of Human SOST.
• Specificity: This antibody recognizes SOST.
• Formulation: PBS
  State: Purified
  State: Liquid purified IgG fraction
  Preservative: 0.09% (W/V) Sodium Azide
• Concentration: lot specific
• Purification: Protein A column, followed by peptide affinity purification
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: sclerostin
• Database Link:
  Entrez Gene 50964 Human
  UniProt ID Q9BQB4
• Background: Sclerostin is a secreted glycoprotein with a C-terminal cysteine knot-like (CTCK) domain and sequence similarity to the DAN (differential screening-selected gene aberrative in neuroblastoma) family of bone morphogenetic protein (BMP) antagonists. Loss-of-function mutations in this gene are associated with an autosomal-recessive disorder, sclerosteosis, which causes progressive bone overgrowth. A deletion downstream of this gene, which causes reduced sclerostin expression, is associated with a milder form of the disorder called van Buchem disease.
• Synonyms: sclerosteosis; sclerostin; VBCH