Sclerostin (SOST) (Center) Rabbit Polyclonal Antibody

CAT#: AP13236PU-N

Sclerostin (SOST) (Center) rabbit polyclonal antibody, Purified

Product Images

Western blot analysis: SOST Antibody (Center) staining of Mouse lung tissue lysates 35ug/lane). SOST (arrow) was detected using the purified Pab.

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  Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with SOST antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: ELISA: 1/1,000.
  Western Blot: 1/50-1/100
  Immunohistochemistry: 1/10-1/50.
• Reactivities: Human
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: KLH conjugated synthetic peptide between 141~171 amino acids from the Center region of Human SOST.
• Specificity: This antibody recognizes SOST (Center).
• Formulation: PBS with 0.09% (W/V) Sodium Azide
  State: Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Protein A Chromatography, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS
• Conjugation: Unconjugated
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: sclerostin
• Database Link:
  Entrez Gene 50964 Human
  UniProt ID Q9BQB4
• Background: Sclerostin is a secreted glycoprotein with a C-terminal cysteine knot-like (CTCK) domain and sequence similarity to the DAN (differential screening-selected gene aberrative in neuroblastoma) family of bone morphogenetic protein (BMP) antagonists. Loss-of-function mutations in this gene are associated with an autosomal-recessive disorder, sclerosteosis, which causes progressive bone overgrowth. A deletion downstream of the sclerostin gene, which causes reduced sclerostin expression, is associated with a milder form of the disorder called van Buchem disease.
• Synonyms: sclerosteosis; sclerostin; VBCH