Ilk (C-term) Rabbit Polyclonal Antibody

CAT#: AP13652PU-N

Ilk (C-term) rabbit polyclonal antibody, Purified

Product Images

(LEFT) Western blot analysis of anti-ILK2/ILK1 Antibody (C-term) in Hela cell line lysates (35ug/lane). ILK2 (arrow) was detected using the purified Pab. (RIGHT) Western blot analysis of anti-ILK2/ILK1 Antibody (C-term) in mouse heart tissue lysates (35ug/lane). ILK2 (arrow) was detected using the purified Pab.

Specifications

Product Data

• Applications: WB
• Recommended Dilution: ELISA: 1/1,000.
  Western blotting: 1/100 - 1/500.
  Flow cytometry.
• Reactivities: Human, Mouse
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human ILK2/ILK1.
• Specificity: This antibody reacts to ILK2/ILK1.
• Formulation: PBS with 0.09% (W/V) sodium azide
  State: Purified
  State: Liquid purified Ig
• Concentration: lot specific
• Purification: Protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: Mus musculus integrin linked kinase (Ilk), transcript variant 2
• Database Link:
  Entrez Gene 3611 HumanEntrez Gene 16202 Mouse
• Background: Transduction of extracellular matrix signals through integrins influences intracellular and extracellular functions, and appears to require interaction of integrin cytoplasmic domains with cellular proteins. Integrin-linked kinase (ILK) is an ankyrin repeat containing 51 kDa receptor-proximate serine-threonine kinase (1), with a reported migration rate of 59K. This 451 amino acid protein interacts with the cytoplasmic domain of the beta-1 integrin subunit and contains sequence motifs found in pleckstrin homology domains capable of interacting with phosphoinositide lipids. ILK is an upstream regulator of Pi(3)K dependant activation of protein kinase B (PKB/AKT) and inhibition of glycogen synthase kinase 3 (GSK-3). ILK2 expression is associated with mediation of cell architecture, adhesion to integrin substrates and anchorage-dependent growth in epithelial cells. ILK2 is overexpressed in some highly invasive tumor cell lines.
• Synonyms: ILK-1, ILK-2, p59ILK, ILK, ILK1, ILK2