JNK1 (MAPK8) (C-term) Rabbit Polyclonal Antibody

CAT#: AP14120PU-N

JNK1 (MAPK8) (C-term) rabbit polyclonal antibody, Purified

Product Images

Western blot analysis of anti-JNK1 Pab in HL60 (UV-treated) cell lysate. JNK1 (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.

Specifications

Product Data

• Applications: WB
• Recommended Dilution: ELISA: 1/1,000.
  Western blotting: 1/100 - 1/500.
  Flow cytometry.
• Reactivities: Human
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human JNK1.
• Specificity: This antibody reacts to JNK1.
• Formulation: PBS with 0.09% (W/V) sodium azide
  State: Purified
  State: Liquid purified Ig
• Concentration: lot specific
• Purification: Protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: mitogen-activated protein kinase 8
• Database Link:
  Entrez Gene 5599 Human
• Background: JNK1 is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is activated by various cell stimuli, and targets specific transcription factors, and thus mediates immediate-early gene expression in response to cell stimuli. The activation of this kinase by tumor-necrosis factor alpha (TNF-alpha) is found to be required for TNF-alpha induced apoptosis. This kinase is also involved in UV radiation induced apoptosis, which is thought to be related to cytochrom c-mediated cell death pathway. Studies of the mouse counterpart of this gene suggested that this kinase play a key role in T cell proliferation, apoptosis and differentiation.
• Synonyms: Mitogen-activated protein kinase 8, c-Jun N-terminal kinase 1, JNK-46, JNK-1, PRKM8