CYP1A1 (C-term) Rabbit Polyclonal Antibody

CAT#: AP14897PU-N

CYP1A1 (C-term) rabbit polyclonal antibody, Purified

Product Images

Figure 1. (LEFT) Western blot analysis of anti-CYP1A1 Antibody (C-term) in K562 cell line lysates (35ug/lane). CYP1A1 (arrow) was detected using the purified Pab. (RIGHT) Western blot analysis of anti-CYP1A1 Antibody (C-term) in mouse lung tissue lysates (35ug/lane). CYP1A1 (arrow) was detected using the purified Pab.

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  Figure 2. Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with CYP1A1 antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: ELISA: 1/1,000.
  Western blotting: 1/50-1/100.
  Immunohistochemistry: 1/50-1/100.
• Reactivities: Human, Mouse
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: KLH conjugated synthetic peptide between 384~414 amino acids from the C-terminal region of Human CYP1A1
• Specificity: This antibody is specific to CYP1A1 (C-term).
• Formulation: PBS with 0.09% (W/V) Sodium Azide as preservative.
  State: Purified
  State: Liquid purified Ig fraction.
• Concentration: lot specific
• Purification: Prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: cytochrome P450 family 1 subfamily A member 1
• Database Link:
  Entrez Gene 1543 Human
• Background: CYP1A1 is a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and its expression is induced by some polycyclic aromatic hydrocarbons (PAHs), some of which are found in cigarette smoke. The enzyme's endogenous substrate is unknown; however, it is able to metabolize some PAHs to carcinogenic intermediates. CYP1A1 gene has been associated with lung cancer risk.
• Synonyms: Cytochrome P450 1A1, Cytochrome P450-P1, Cytochrome P450 form 6, Cytochrome P450-C
• Note: Calculated Molecular Weight: 58165 kDa