MARK2 (C-term) Rabbit Polyclonal Antibody

CAT#: AP14909PU-N

MARK2 (C-term) rabbit polyclonal antibody, Purified

Product Images

(LEFT) Western blot analysis of anti-EMK Antibody (C-term)in HepG2 cell line lysates. EMK-V581 (arrow) was detected using the purified Pab. (RIGHT) Western blot analysis of anti-EMK Antibody (C-term) in mouse thymus tissue lysates (35ug/lane). EMK (arrow) was detected using the purified Pab.

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  Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: ELISA: 1/1,000.
  Immunohistochemistry: 1/50 - 1/100.
  Western Blot: 1/250 - 1/500.
• Reactivities: Human, Mouse
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human EMK.
• Specificity: This antibody reacts to MARK2 (EMK).
• Formulation: PBS with 0.09% (W/V) sodium azide
  State: Purified
  State: Liquid purified Ig
• Concentration: lot specific
• Purification: Protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: Homo sapiens MAP/microtubule affinity-regulating kinase 2 (MARK2), transcript variant 3
• Database Link:
  Entrez Gene 13728 MouseEntrez Gene 2011 Human
• Background: Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains.
• Synonyms: Serine/threonine-protein kinase, 2, ELKL motif kinase, EMK1, PAR1 homolog, MARK2, EMK1

Reference Data

• Protein Families: Druggable Genome, Protein Kinase