FKBP52 (FKBP4) Rabbit Polyclonal Antibody

CAT#: AP17384PU-N

FKBP52 (FKBP4) rabbit polyclonal antibody, Purified

Product Images

Western blot analysis of FKBP4 Antibody (Center) in Hela, MCF7, T47D cell line lysates (35 ug/lane). FKBP4 (arrow) was detected using the purified Pab.

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  Western blot analysis of FKBP4 (arrow) using FKBP4 Antibody (Center). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the FKBP4 gene.

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  Flow cytometric analysis of MCF-7 cells using FKBP4 Antibody (Center) (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Specifications

Product Data

• Applications: FC, WB
• Recommended Dilution: ELISA: 1/1,000.
  Western blot: 1/1000.
  Flow Cytometry: 1/10-1/50.
• Reactivities: Human
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: KLH conjugated synthetic peptide between 186-216 amino acids from the Central region of Human FKBP4.
• Specificity: This antibody detects FKBP4 at Center.
• Formulation: PBS with 0.09% (W/V) Sodium Azide
  State: Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 51805 Da
• Gene Name: Homo sapiens FK506 binding protein 4 (FKBP4)
• Database Link:
  Entrez Gene 2288 Human
• Background: FKBP4 is a member of the immunophilin protein family, which play a role in immunoregulation and basic cellular processes involving protein folding and trafficking. This protein is a cis-trans prolyl isomerase that binds to the immunosuppressants FK506 and rapamycin. It has high structural and functional similarity to FK506-binding protein 1A (FKBP1A), but unlike FKBP1A, this protein does not have immunosuppressant activity when complexed with FK506. It interacts with interferon regulatory factor-4 and plays an important role in immunoregulatory gene expression in B and T lymphocytes. This protein is known to associate with phytanoyl-CoA alpha-hydroxylase. It can also associate with two heat shock proteins (hsp90 and hsp70) and thus may play a role in the intracellular trafficking of hetero-oligomeric forms of the steroid hormone receptors. This protein correlates strongly with adeno-associated virus type 2 vectors (AAV) resulting in a significant increase in AAV-mediated transgene expression in human cell lines. Thus this protein is thought to have important implications for the optimal use of AAV vectors in human gene therapy.
• Synonyms: FK506-binding protein 4, FKBP52 protein, FKBP59, p59 protein

Reference Data

• Protein Families: Druggable Genome