Galactosidase alpha (GLA) (N-term) Rabbit Polyclonal Antibody

CAT#: AP17424PU-N

Galactosidase alpha (GLA) (N-term) rabbit polyclonal antibody

Product Images

Western blot analysis of GLA antibody (N-term) in Hela cell line lysates (35 ug/lane). GLA (arrow) was detected using the purified Pab.

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  GLA Antibody (N-term) IHC analysis in formalin fixed and paraffin embedded human Lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the GLA Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: Western blot: 1/50 - 1/100.
  ELISA: 1/1,000.
  Immunohistochemistry on paraffin sections: 1/50 - 1/100.
• Reactivities: Human
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: KLH conjugated synthetic peptide between 90~120 amino acids from the N-terminal region of human GLA
• Specificity: This antibody detects GLA at N-term.
• Formulation: PBS with 0.09% (W/V) sodium azide as preservative
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS
• Storage: Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: Homo sapiens galactosidase alpha (GLA)
• Database Link:
  Entrez Gene 2717 Human
• Background: GLA is a homodimeric glycoprotein that hydrolyses the terminal alpha-galactosyl moieties from glycolipids and glycoproteins. This enzyme predominantly hydrolyzes ceramide trihexoside, and it can catalyze the hydrolysis of melibiose into galactose and glucose. A variety of mutations in this gene affect the synthesis, processing, and stability of this enzyme, which causes Fabry disease, a rare lysosomal storage disorder that results from a failure to catabolize alpha-D-galactosyl glycolipid moieties.
• Synonyms: Alpha-D-galactosidase A, Melibiase
• Note: Molecular weight: 48767 Da

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Galactose metabolism, Glycerolipid metabolism, Glycosphingolipid biosynthesis - globo series, Lysosome, Sphingolipid metabolism