PRAME (C-term) Rabbit Polyclonal Antibody

CAT#: AP17546PU-N

PRAME (C-term) rabbit polyclonal antibody

Product Images

Western blot analysis of Mrified Pab.

Specifications

Product Data

• Applications: FC, IHC, WB
• Recommended Dilution: Peptide ELISA: 1/1,000.
  Flow Cytometry: 1/25.
  Western blotting: 1/2,000.
  Immunohistochemistry on Paraffin Sections: 1/50-1/100.
• Reactivities: Human
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: KLH conjugated synthetic peptide between 476-502 amino acids from the C-terminal region of human MAPE.
• Specificity: This antibody reacts to MAPE.
• Formulation: PBS with 0.09% (W/V) sodium azide
  State: Liquid purified Ig
• Concentration: lot specific
• Purification: Saturated Ammonium Sulfate (SAS) precipitation
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: Homo sapiens preferentially expressed antigen in melanoma (PRAME), transcript variant 1
• Database Link:
  Entrez Gene 23532 Human
• Background: MAPE is an antigen that is predominantly expressed in human melanomas and that is recognized by cytolytic T lymphocytes. It is not expressed in normal tissues, except testis. This expression pattern is similar to that of other CT antigens, such as MAGE, BAGE and GAGE. However, unlike these other CT antigens, its gene is also expressed in acute leukemias.
• Synonyms: OPA-interacting protein 4, OIP4, OIP-4