VDAC1 (N-term) Rabbit Polyclonal Antibody

CAT#: AP17825PU-N

VDAC1 (N-term) rabbit polyclonal antibody, Purified

Product Images

Western blot analysis of VDAC1 Antibody (N-term) in A375 cell line and mouse heart tissue lysates (35ug/lane). VDAC1 (arrow) was detected using the purified Pab.

Specifications

Product Data

• Applications: FC, IHC, WB
• Recommended Dilution: Peptide ELISA: 1/1,000.
  Western blotting: 1/1,000.
  Flow cytometry: 1/10-1/50.
  Immunohistochemistry on Paraffin Sections: 1/50-1/100.
• Reactivities: Human, Mouse
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human VDAC1.
• Specificity: This antibody recognizes VDAC-1.
• Formulation: PBS with 0.09% (W/V) Sodium Azide as preservative.
  State: Purified
  State: Liquid purified Ig fraction.
• Concentration: lot specific
• Purification: Purified through a protein A column, followed by peptide affinity purification
• Conjugation: Unconjugated
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: voltage dependent anion channel 1
• Database Link:
  Entrez Gene 22333 MouseEntrez Gene 7416 Human
  UniProt ID P21796
• Background: VDAC1 forms a channel through the mitochondrial outer membrane and also the plasma membrane. The channel at the outer mitochondrial membrane allows diffusion of small hydrophilic molecules; in the plasma membrane it is involved in cell volume regulation and apoptosis. It adopts an open conformation at low or zero membrane potential and a closed conformation at potentials above 30-40 mV. The open state has a weak anion selectivity whereas the closed state is cation-selective. The protein may participate in the formation of the permeability transition pore complex (PTPC) responsible for the release of mitochondrial products that triggers apoptosis.
• Synonyms: VDAC, Porin 31HL, Porin 31HM, Mitochondria marker
• Note: Calculated MW: 30773 Da

Reference Data

• Protein Families: Ion Channels: Other
• Protein Pathways: Calcium signaling pathway, Huntington's disease, Parkinson's disease