Villin (VIL1) (N-term) Rabbit Polyclonal Antibody

CAT#: AP17828PU-N

Villin (VIL1) (N-term) rabbit polyclonal antibody

Product Images

Western blot analysis of Villin-1 Antibody (N-term) in mouse kidney tissue lysates (35ug/lane). VIL1 (arrow) was detected using the purified Pab.

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  Formalin-fixed and paraffin-embedded human colon carcinoma reacted with Villin-1 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

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  Flow Cytometric analysis of WiDr cells using Villin-1 Antibody (N-term) (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Specifications

Product Data

• Applications: FC, IHC, WB
• Recommended Dilution: ELISA: 1/1,000.
  Western blotting: 1/1,000.
  Flow Cytometry: 1/10-1/50.
  Immunohistochemistry on Paraffin Sections: 1/50-1/100.
• Reactivities: Human, Mouse
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: KLH conjugated synthetic peptide between 180-207 amino acids from the N-terminal region of Human Villin-1.
• Specificity: This antibody recognizes Villin-1.
• Formulation: PBS
  State: Liquid purified Ig fraction
  Preservative: 0.09% (W/V) Sodium Azide
• Concentration: lot specific
• Purification: Purified through a protein A column, followed by peptide affinity purification.
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: villin 1
• Database Link:
  Entrez Gene 22349 MouseEntrez Gene 7429 Human
  UniProt ID P09327
• Background: Villin-1 is a member of a family of calcium-regulated actin-binding proteins. This protein represents a dominant part of the brush border cytoskeleton which functions in the capping, severing, and bundling of actin filaments.
• Synonyms: VIL1