ZWINT (Center) Rabbit Polyclonal Antibody

CAT#: AP17856PU-N

ZWINT (Center) rabbit polyclonal antibody, Purified

Product Images

Western blot analysis: ZWINT Antibody staining of Mouse cerebellum tissue lysates (35ug/lane). ZWINT (arrow) was detected using the purified Pab.

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  Western blot analysis: ZWINT Antibody staining of 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the ZWINT gene.

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  Immunohitochemistry: ZWINT Antibody staining of Formalin-Fixed, Paraffin-Embedded Human Brain Tissue. This ZWINT antibody (Center) was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. Clinical relevance has not been evaluated.

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  Flow Cytometry: Analysis of widr cells using ZWINT Antibody (Center) (bottom histogram) compared to a Negative control cell (Top histogram). FITC-conjugated Goat-anti-Rabbit secondary antibodies were used for the analysis.

Specifications

Product Data

• Applications: FC, IHC, WB
• Recommended Dilution: ELISA: 1/1000.
  Western blotting: 1/1000.
  Flow Cytometry: 1/10-1/50.
  Immunohistochemistry: 1/50-1/100.
• Reactivities: Human, Mouse
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: KLH conjugated synthetic peptide between 59-88 selected from the Center region of Human ZWINT
• Specificity: This antibody recognizes ZW10 interactor/ZWINT (Center).
• Formulation: PBS
  State: Purified
  State: Liquid purified Ig fraction.
  Preservative: 0.09% (W/V) Sodium Azide
• Concentration: lot specific
• Purification: Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: Homo sapiens ZW10 interacting kinetochore protein (ZWINT), transcript variant 3
• Database Link:
  Entrez Gene 11130 Human
• Background: ZWINT is clearly involved in kinetochore function although an exact role is not known. It interacts with ZW10, another kinetochore protein, possibly regulating the association between ZW10 and kinetochores. The protein localizes to prophase kinetochores before ZW10 does and it remains detectable on the kinetochore until late anaphase. It has a uniform distribution in the cytoplasm of interphase cells.
• Synonyms: Zwint-1
• Note: Calculated MW: 31293 Da

Reference Data

• Protein Families: Druggable Genome