CARD4 (NOD1) (C-term) Rabbit Polyclonal Antibody

CAT#: AP17920PU-N

CARD4 (NOD1) (C-term) rabbit polyclonal antibody, Purified

Product Images

Western blot analysis of CARD4 Antibody (C-term) in HL-60 cell line lysates (35ug/lane). CARD4 (arrow) was detected using the purified Pab.

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  CARD4 antibody staining of Formalin-Fixed, Paraffin-Embedded Human skeletal muscle tissue. This Antibody was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: ELISA: 1/1,000.
  Western blotting: 1/100-1/500.
  Immunohistochemistry: 1/50-1/100.
• Reactivities: Human
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: KLH conjugated synthetic peptide selected from the C-terminal region of Human CARD4.
• Specificity: This antibody recognizes CARD4 / NOD1 (C-term).
• Formulation: PBS with 0.09% (W/V) Sodium Azide as preservative.
  State: Purified
  State: Liquid purified Ig fraction.
• Concentration: lot specific
• Purification: Affinity Chromatography on Protein A.
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: Homo sapiens nucleotide binding oligomerization domain containing 1 (NOD1)
• Database Link:
  Entrez Gene 10392 Human
• Background: CARD4 is a member of the NOD (nucleotide-binding oligomerization domain) family. This member is a cytosolic protein. It contains an N-terminal caspase recruitment domain (CARD), a centrally located nucleotide-binding domain (NBD), and 10 tandem leucine-rich repeats (LRRs) in its C terminus. The CARD is involved in apoptotic signaling, LRRs participate in protein-protein interactions, and mutations in the NBD may affect the process of oligomerization and subsequent function of the LRR domain. This protein is an intracellular pattern-recognition receptor (PRR) that initiates inflammation in response to a subset of bacteria through the detection of bacterial diaminopimelic acid.
• Synonyms: CARD4; CLR7.1; NLRC1
• Note: Calculated Molecular Weight: 107691 Da

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Epithelial cell signaling in Helicobacter pylori infection, NOD-like receptor signaling pathway