HSPC142 (BABAM1) Rabbit Polyclonal Antibody

CAT#: AP18061PU-N

HSPC142 (BABAM1) rabbit polyclonal antibody

Product Images

Figure 1. Western blot analysis of HSPC142 Antibody (Center) in MDA-MB231 cell line lysates (35ug/lane). HSPC142 (arrow) was detected using the purified Pab.

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  Figure 2. Formalin-fixed and paraffin-embedded human brain reacted with HSPC142 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: ELISA: 1/1,000.
  Western blotting: 1/1,000.
  Immunohistochemistry: 1/50-1/100.
• Reactivities: Human
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: KLH conjugated synthetic peptide between 116-143 amino acids from the Central region of Human HSPC142.
• Specificity: This antibody recognizes MERIT40/HSPC142 (Center).
• Formulation: PBS with 0.09% (W/V) Sodium Azide as preservative.
  State: Liquid purified Ig fraction.
• Concentration: lot specific
• Purification: Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: Homo sapiens BRISC and BRCA1 A complex member 1 (BABAM1), transcript variant 1
• Database Link:
  Entrez Gene 29086 Human
• Background: Component of the BRCA1-A complex, a complex that specifically recognizes 'Lys-63'-linked ubiquitinated histones H2A and H2AX at DNA lesions sites, leading to target the BRCA1-BARD1 heterodimer to sites of DNA damage at double-strand breaks (DSBs). The BRCA1-A complex also possesses deubiquitinase activity that specifically removes 'Lys-63'-linked ubiquitin on histones H2A and H2AX. In the BRCA1-A complex, it is required for the complex integrity and its localization at DSBs. Probably also plays a role as a component of the BRISC complex, a multiprotein complex that specifically cleaves 'Lys-63'-linked ubiquitin. In these 2 complexes, it is probably required to maintain the stability of BRE/BRCC45 and help the 'Lys-63'-linked deubiquitinase activity mediated by BRCC3/BRCC36. component.
• Synonyms: C19orf62, NBA1, HSPC142
• Note: Calculated MW: 36560 Da