SC35 (SRSF2) (Center) Rabbit Polyclonal Antibody

CAT#: AP18194PU-N

SC35 (SRSF2) (Center) rabbit polyclonal antibody

Product Images

Western blot analysis of SFRS2 Antibody (Center) in mouse stomach tissue lysates (35ug/lane). SFRS2 (arrow) was detected using the purified Pab.

Specifications

Product Data

• Applications: WB
• Recommended Dilution: ELISA: 1/1,000.
  Western blotting: 1/50 - 1/100.
• Reactivities: Human
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: KLH conjugated synthetic peptide selected from aa 83-113 from the Center region of human SFRS2
• Specificity: This antibody reacts to SFRS2.
• Formulation: PBS with 0.09% (W/V) sodium azide
  State: Liquid purified Ig
• Concentration: lot specific
• Purification: Saturated Ammonium Sulfate (SAS) precipitation
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: Homo sapiens serine and arginine rich splicing factor 2 (SRSF2), transcript variant 1
• Database Link:
  Entrez Gene 6427 Human
• Background: SFRS2 is necessary for the splicing of pre-mRNA. The protein is required for formation of the earliest ATP-dependent splicing complex and interacts with spliceosomal components bound to both the 5'- and 3'-splice sites during spliceosome assembly. It also is required for ATP-dependent interactions of both U1 and U2 snRNPs with pre-mRNA. And it interacts with other spliceosomal components, via the RS domains, to form a bridge between the 5'- and 3'-splice site binding components, U1 snRNP and U2AF. It binds to purine-rich RNA sequences, either 5'-AGSAGAGTA-3' (S=C or G) or 5'-GTTCGAGTA-3' and can bind to beta-globin mRNA and commit it to the splicing pathway.
• Synonyms: Splicing factor SC35, Splicing component 35 kDa, PR264, Nuclear Speckle Marker

Reference Data

• Protein Families: Stem cell - Pluripotency, Transcription Factors
• Protein Pathways: Spliceosome