TOB (TOB1) (N-term) Rabbit Polyclonal Antibody

CAT#: AP18225PU-N

TOB (TOB1) (N-term) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot analysis of TOB1 Antibody (N-term) in Mouse liver tissue lysates (35ug/lane). TOB1 (arrow) was detected using the purified Pab.

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  Formalin-Fixed, Paraffin-Eembedded Human hepatocarcinoma reacted with TOB1 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

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  Flow Cytometric analysis of NCI-H292 cells using TOB1 Antibody (N-term) Flow (Bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated Goat-anti-Rabbit secondary antibodies were used for the analysis.

Specifications

Product Data

• Applications: FC, IHC, WB
• Recommended Dilution: ELISA: 1/1,000.
  Western blotting: 1/100-1/500.
  Flow Cytometry: 1/10-1/50.
  Immunohistochemistry: 1/50-1/100.
• Reactivities: Human, Mouse
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: KLH conjugated synthetic peptide between 61~91 amino acids from the N-terminal region of Human TOB1
• Specificity: This antibody recognizes TOB1 (N-term).
• Formulation: PBS with 0.09% (W/V) Sodium Azide as preservative
  State: Aff - Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Affinity Chromatography on Protein A
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: Homo sapiens transducer of ERBB2, 1 (TOB1), transcript variant 1
• Database Link:
  Entrez Gene 22057 MouseEntrez Gene 10140 Human
• Background: TOB1 is a member of the tob/btg1 family of anti-proliferative proteins that have the potential to regulate cell growth. When exogenously expressed, this protein supresses cell growth in tissue culture. The protein undergoes phophorylation by a serine/threonine kinase, 90 kDa ribosomal S6 kinase. Interactions of this protein with the v-erb-b2 erythroblastic leukemia viral oncogene homolog 2 gene product p185 interferes with growth suppression. This protein inhibits T cell proliferation and transcription of cytokines and cyclins. The protein interacts with both mothers against decapentaplegic Drosophila homolog 2 and 4 to enhance their DNA binding activity. This interaction inhibits interleukin 2 transcription in T cells.
• Synonyms: TOB, TROB1
• Note: Molecular Weight: 38155 Da

Reference Data

• Protein Families: Druggable Genome