uidA Rabbit Polyclonal Antibody

CAT#: AP20094BT-N

uidA rabbit polyclonal antibody, Biotin


USD 410.00

2 Weeks*

Size
    • 1 ml

Product Images

Specifications

Product Data
Applications ELISA, ID, IF, IP, R, WB
Recommended Dilution This product is intended for use in precipitating and non-precipitating antibody-binding assays such as e.g., ELISA and Western blotting and Immunofluorescence or Histochemical techniques.
Recommended Working Dilutions:
Non-precipitating antibody-binding techniques: 1/1,000-1/10,000.
Reactivities Escherichia coli
Host Rabbit
Isotype IgG
Clonality Polyclonal
Immunogen Beta-Glucuronidase is isolated and purified from Escherichia coli.
Freund’s complete adjuvant is used in the first step of the immunization procedure.
Specificity The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: Immunoelectrophoresis, Cross-Immunoelectrophoresis, single Radial Immunodiffusion (Ouchterlony), block titration, ELISA, Immunoblotting and Enzyme Inhibition.
Cross-reactivities against enzymes of other sources may occur but have not been determined.
Formulation PBS, pH 7.2 without preservatives and foreign proteins.
Label: Biotin
State: Lyophilized IgG fraction.
Label: Conjugation Procedure: A proprietary technique for the binding to biotin is used, followed by several purification steps. After each step activity and specificity are tested in a variety of techniques.
The conjugate is lyophilized to assure stability and long shelf life.
Molar radio: Biotin/IgG~ 6.2
Reconstitution Method Restore by adding 1.0 ml of sterile distilled water.
Concentration 10.0 mg/ml
Purification Ammonium Sulphate Precipitation and Ion Exchange Chromatography.
Conjugation Biotin
Background Reporter genes are widely used for studying the expression of foreign genes in transformed plants tissues. Using appropriate promoter-reporter gene constructs, this technique allows an independent verification of the transformed status of tissues growing on media containing selective antibiotics or herbicides. In addition, it serves as a principal means to follow gene transfer and monitor genetic transformation of plant species. Encoded by the E. coli GUS gene (also referred to as uidA), GUS protein is a hydrolase that catalyses the cleavage of a variety of beta-glucuronide derivatives available for colorimetric, fluorimetric and histochemical assays. Several features make the gus gene superior as a reporter gene for plant studies and in the production of genetically engineered crops.
Synonyms GUSB, Beta-G1
Reference Data

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.