ald Rabbit Polyclonal Antibody

CAT#: AP20099BT-N

ald rabbit polyclonal antibody, Biotin

Specifications

Product Data

• Applications: ELISA, ID, IF, IP, R, WB
• Recommended Dilution: This product is intended for use in precipitating and non-precipitating antibody-binding assays such as e.g., ELISA and Western blotting and Immunofluorescence or Histochemical techniques (1/1,000-1/10,000).
• Reactivities: Bacillus sp.
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: L-Alanine Dehydrogenase isolated and purified from Bacillus subtilis.
  Freund's complete adjuvant is used in the first step of the immunization procedure.
• Specificity: The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: Immunoelectrophoresis, Cross-Immunoelectrophoresis, single Radial Immunodiffusion (Ouchterlony), block titration, ELISA, Immunoblotting and Enzyme Inhibition.
  Cross-reactivities against enzymes of other sources may occur but have not been determined.
  Recognizes L-Alanine Dehydrogenase.
• Formulation: PBS, pH 7.2 without preservatives and foreign proteins.
  Label: Biotin
  State: Lyophilized IgG fraction.
  Label: Conjugation Procedure: A proprietary technique for the binding to biotin is used, followed by several purification steps. After each step activity and specificity are tested in a variety of techniques. The conjugate is lyophilized to assure stability and long shelf life
  Molar radio: ~5.4
• Reconstitution Method: Restore by adding 1.0 ml of sterile distilled water.
• Concentration: 10.0 mg/ml
• Purification: Ammonium Sulphate Precipitation and Ion Exchange Chromatography.
• Conjugation: Biotin
• Background: This enzyme is a key factor in the assimilation of L-alanine as an energy source through the tricarboxylic acid cycle during sporulation.
• Synonyms: ald, spoVN, BSU31930