ALDOA Sheep Polyclonal Antibody

CAT#: AP20105BT-N

ALDOA sheep polyclonal antibody, Biotin


USD 410.00

2 Weeks*

Size
    • 1 ml

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Specifications

Product Data
Applications ELISA, ID, IF, IP, R, WB
Recommended Dilution This product is intended for use in precipitating and non-precipitating antibody-binding assays such as e.g., ELISA and Western blotting and Immunofluorescence or Histochemical techniques (1/200-1/1,000).
Reactivities Rabbit
Host Sheep
Isotype IgG
Clonality Polyclonal
Immunogen Aldolase isolated and purified from Rabbit muscle.
Freund's complete adjuvant is used in the first step of the immunization procedure.
Specificity The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: Immunoelectrophoresis, Cross-Immunoelectrophoresis, single Radial Immunodiffusion (Ouchterlony), block titration, ELISA, Immunoblotting and Enzyme Inhibition.
Cross-reactivities against enzymes of other sources may occur but have not been determined.
Recognizes Aldolase.
Formulation PBS, pH 7.2 without preservatives and foreign proteins.
Label: Biotin
State: Lyophilized IgG fraction.
Label: Conjugation Procedure: A proprietary technique for the binding to biotin is used, followed by several purification steps. After each step activity and specificity are tested in a variety of techniques. The conjugate is lyophilized to assure stability and long shelf life
Molar radio: ~8.1
Reconstitution Method Restore by adding 1.0 ml of sterile distilled water.
Concentration 10.0 mg/ml
Purification Ammonium Sulphate Precipitation and Ion Exchange Chromatography.
Conjugation Biotin
Background This gene product, Aldolase A (fructose bisphosphate aldolase) is a glycolytic enzyme that catalyzes the reversible conversion of fructose-1,6-bisphosphate to glyceraldehyde 3 phosphate and dihydroxyacetone phosphate. Three aldolase isozymes (A, B, and C), encoded by three different genes, are differentially expressed during development. Aldolase A is found in the developing embryo and is produced in even greater amounts in adult muscle. Aldolase A expression is repressed in adult liver, kidney and intestine and similar to aldolase C levels in brain and other nervous tissue. Aldolase A deficiency has been associated with myopathy and hemolytic anemia. Alternative splicing of this gene results in multiple transcript variants which encode the same protein.
Synonyms ALDA, NY-LU-1, Muscle-type aldolase
Reference Data

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