PIN1 Chicken Polyclonal Antibody

CAT#: AP20127SU-N

PIN1 chicken polyclonal antibody, Serum

Product Images

AP20127SU-N PIN1 antibody staining of HeLa cells at 1/1,000 dilution (Green). Fibrillarin antibody (Red). Pin-1 stains the nuclear matrix and, much more faintly, the cytoplasm. The Fibrillarin antibody marks nucleoli.

Specifications

Product Data

• Applications: IF, WB
• Recommended Dilution: Western Blot: 1/500-1/10,000
  Immunofluorescence: 1/1,000
  Immunocytochemistry: 1/5,000 (ABC or other enzyme linked immunocytochemical procedures).
• Reactivities: Human
• Host: Chicken
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: Recombinant full length Pin-1 purified from E. coli.
• Specificity: PIN1
• Formulation: PBS with 10mM Sodium Azide as a preservative.
  State: Serum
  State: Liquid Whole Serum
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C to -70°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: Homo sapiens peptidylprolyl cis/trans isomerase, NIMA-interacting 1 (PIN1), transcript variant 1
• Database Link:
  Entrez Gene 5300 Human
• Background: Pin-1 is a the peptidylprolyl cis/trans isomerase enzyme which is responsible, as its name suggests, for flipping the proline ring from the cis to the trans conformation.
  Pin1 has been implicated in tau pathologies that underlie Alzheimer's Disease. Pin1 binds to tau phosphorylated specifically on the Thr231-Pro site and induces conformational changes in tau. Such conformational changes can directly restore the ability of phosphorylated Tau to bind microtubules and promote microtubule assembly and/or facilitate tau dephosphorylation. Pin1 expression inversely correlates with the predicted neuronal vulnerability in normally aged brain and also with actual neurofibrillary degeneration in AD brain. Pin1 could be pivotal for maintainance of normal neuronal function and preventing age-dependent neurodegeneration. It is also heavily upregulated in tumor cells, so that antibodies to this protein can be used as tumor markers.
• Synonyms: PIN-1

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: RIG-I-like receptor signaling pathway