CYP7B1 (C-term) Goat Polyclonal Antibody

CAT#: AP20144PU-N

CYP7B1 (C-term) goat polyclonal antibody, Aff - Purified

Product Images

Immunohistochemistry: CYP7B1 Antibody staining of Paraffin Embedded Human Breast at 4 ug/ml. Steamed antigen retrieval with citrate buffer pH 6, HRP-

Specifications

Product Data

• Applications: ELISA, IHC
• Recommended Dilution: Peptide ELISA: Detection Limit: 1/128000.
  Western blot: Preliminary experiments in Human Liver, Kidney and Prostate lysates gave no specific signal but low background (at antibody concentration up to 1µg/ml).
  Immunohistochemistry on Paraffin Sections: 3-6 µg/ml.
  In Paraffin Embedded Human Breast shows textured Cytoplasm staining in ductal epithelial cells.
• Reactivities: Bovine, Human
• Host: Goat
• Clonality: Polyclonal
• Immunogen: Peptide with sequence C-YPDSDVLFRYKVKS, from the C Terminus of the protein sequence according to NP_004811.1.
• Specificity: This antibody recognizes CYP7B1.
• Formulation: Tris saline, pH~7.3
  State: Aff - Purified
  State: Liquid purified IgG fraction.
  Stabilizer: 0.5% BSA
  Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purification: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide.
• Gene Name: Homo sapiens cytochrome P450 family 7 subfamily B member 1 (CYP7B1), transcript variant 1
• Database Link:
  Entrez Gene 9420 Human
• Background: P450 enzymes constitute a family of monooxygenase enzymes that are involved in the metabolism of a wide array of endogenous and xenobiotic compounds including cholesterol. CYP8B1 moderates the ratio of cholic acid over chenodeoxycholic acid to control the solubility of cholesterol.
  P450 cholesterol 7-hydroxylase, CYP7A1, is the rate limiting enzyme of bile acid synthesis in the liver, and its expression is mediated by the bile acid receptor FXR. CYP27A1 catalyzes vitamin D3 25-hydroxylation and is localized to the mitochondria in kidney and liver. CYP7B1 (oxysterol 7-α-hydroxylase) functions as an enzyme in the alternate bile acid synthesis pathway. Specifically, CYP7B1 metabolizes 25-and 27-hydroxycholesterol. The gene encoding human CYP7B1 maps to chromosome 8q21.3. Mutations in the CYP7B1 gene may cause a metabolic defect in bile acid synthesis characterized by elevated urinary bile acid excretion, severe cholestasis, cirrhosis and liver synthetic failure.
• Synonyms: Cytochrome P450 7B1

Reference Data

• Protein Families: Druggable Genome, P450, Transmembrane
• Protein Pathways: Primary bile acid biosynthesis