Synaptophysin (SYP) Rabbit Polyclonal Antibody

CAT#: AP20199PU-N

Synaptophysin (SYP) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot (WB) analysis of SYP antibody (Cat.-No.: AP20199PU-N) in extracts from LOVO cells.

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  Immunohistochemistry Analysis: AP20199PU-N SYP antibody staining of Paraffin-Embedded Human brain tissue.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: Western blot: 1/500-1/1000.
  Immunohistochemistry on paraffin sections: 1/50-1/200.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Specificity: This antibody antibody detects endogenous levels of Synaptophysin protein.
• Formulation: Phosphate buffered saline (PBS), pH~7.2 containing 0.05% Sodium Azide as preservative.
  State: Aff - Purified
  State: Liquid purified Ig fraction (> 95% pure by SDS-PAGE) .
• Concentration: 1.0 mg/ml
• Purification: Affinity Chromatography using epitope-specific immunogen.
• Conjugation: Unconjugated
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: ~ 38 kDa
• Gene Name: Homo sapiens synaptophysin (SYP)
• Database Link:
  Entrez Gene 20977 MouseEntrez Gene 24804 RatEntrez Gene 6855 Human
  UniProt ID P08247
• Background: Synaptic vesicles participate in a cycle of fusion with the plasma membrane and reformation by endocytosis. Synaptic vesicle protein synaptophysin (SYP) is targeted to early endosomes in transfected fibroblasts and in neuroendocrine cells. SYP is an N-glycosylated intergral membrane protein found in neurons and endocrine cells that associates into hexamers to form a large conductance channel. SYP contains four transmembrane domains and may function as a gap juction-like channel. Membrane cholesterol specfically interacts with SYP to play a role in vesicle formation. Synaptobrevin (VAMP) also binds to SYP and the resultant complex is upregulated during neuronal development, but is absent in exocytosis fusion complex. Thus, the synaptophysin-synaptobrevin complex is not essential for exocytosis, but rather provides a pool of synaptobrevin for exocytosis.
• Synonyms: Neuroendocrine Marker

Reference Data

• Protein Families: Secreted Protein, Transmembrane