Aurora A (AURKA) Rabbit Polyclonal Antibody

CAT#: AP20206PU-N

Aurora A (AURKA) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot (WB) analysis of AurA (pPro282) antibody in extracts from 293 cells.

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  Immunohistochemistry analysis with AurA (pPro282) antibody on paraffin sections.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: Western blot: 1/500-1/1000.
  Immunohistochemistry on paraffin sections.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Synthetic peptide, corresponding to amino acids 251-300 of Human AURKA.
• Specificity: This antibody antibody detects endogenous levels of of AurA protein.
• Formulation: Phosphate buffered saline (PBS), pH~7.2 containing 0.05% Sodium Azide as preservative.
  State: Aff - Purified
  State: Liquid purified Ig fraction (> 95% pure by SDS-PAGE)
• Concentration: 1.0 mg/ml
• Purification: Affinity Chromatography using epitope-specific immunogen.
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: ~ 48 kDa
• Gene Name: Homo sapiens aurora kinase A (AURKA), transcript variant 2
• Database Link:
  Entrez Gene 20878 MouseEntrez Gene 261730 RatEntrez Gene 6790 Human
• Background: Aurora 2/AIK, a cell cycle-regulated serine/threonine protein kinase, is overexpressed in many tumor cell lines. Phosphorylation of threonine 288, which is within the activation loop of the kinase, results in a significant increase in its enzymatic activity. Aurora 2/AIK is regulated by proteasome-dependent degradation, and phosphorylation on Thr288 may target it for degradation during mitosis. Thus, phosphorylation of Aurora 2/AIK at Thr288 is important for both kinase activity and stability.
• Synonyms: AURKA, AIK1, ARK1, AURA, BTAK, STK15, STK6, Aurora/IPL1-related kinase 1, AURORA2

Reference Data

• Protein Families: Druggable Genome, Protein Kinase, Stem cell - Pluripotency
• Protein Pathways: Oocyte meiosis