DUSP1 Rabbit Polyclonal Antibody

CAT#: AP20257PU-N

DUSP1 rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot analysis of MKP1 antibody (AP20257PU-N) in extracts from Jurkat cells.

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  Immunohistochemistry analyzes of MKP1 antibody (AP20257PU-N) in paraffin-embedded human brain tissue.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: Western blot: 1/500-1/1000.
  Immunohistochemistry on paraffin sections: 1/50-1/200.
• Reactivities: Human
• Host: Rabbit
• Clonality: Polyclonal
• Specificity: This antibody detects endogenous levels of MKP1 protein.
• Formulation: Phosphate buffered saline (PBS) with 0.05% sodium azide, approx. pH 7.2
  State: Aff - Purified
  State: Liquid purified Ig fraction
• Concentration: 1.0 mg/ml
• Purification: Affinity-chromatography using epitope-specific immunogen; purity is > 95% (by SDS-PAGE)
• Storage:

  Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Predicted Protein Size: ~ 39 kDa
• Gene Name: Homo sapiens dual specificity phosphatase 1 (DUSP1)
• Database Link:
  Entrez Gene 1843 Human
• Background: The expression of DUSP1 gene is induced in human skin fibroblasts by oxidative/heat stress and growth factors. It specifies a protein with structural features similar to members of the non-receptor-type protein-tyrosine phosphatase family, and which has significant amino-acid sequence similarity to a Tyr/Ser-protein phosphatase encoded by the late gene H1 of vaccinia virus. The bacterially expressed and purified DUSP1 protein has intrinsic phosphatase activity, and specifically inactivates mitogen-activated protein (MAP) kinase in vitro by the concomitant dephosphorylation of both its phosphothreonine and phosphotyrosine residues. Furthermore, it suppresses the activation of MAP kinase by oncogenic ras in extracts of Xenopus oocytes. Thus, DUSP1 may play an important role in the human cellular response to environmental stress as well as in the negative regulation of cellular proliferation.
• Synonyms: CL100, MKP-1, PTPN10, VH1, MAP kinase phosphatase 1

Reference Data

• Protein Families: Druggable Genome, Phosphatase
• Protein Pathways: MAPK signaling pathway