PKC mu (PRKD1) (PKD1/2/3) Rabbit Polyclonal Antibody

CAT#: AP20263PU-N

PKC mu (PRKD1) (PKD1/2/3) rabbit polyclonal antibody, Aff - Purified


USD 370.00

2 Weeks*

Size
    • 100 ug

Product Images

Other products for "PRKD1"

Specifications

Product Data
Applications WB
Recommended Dilution Western blot: 1/500-1/1000.
Reactivities Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Specificity This antibody detects endogenous levels of PKD1/2/3/PKC μ protein.
Formulation Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.2
State: Aff - Purified
State: Liquid purified Ig fraction
Concentration 1.0 mg/ml
Purification Affinity-chromatography using epitope-specific immunogen; purity is > 95% (by SDS-PAGE)
Storage

Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.

Stability Shelf life: one year from despatch.

Predicted Protein Size ~ 100 to 140 kDa
Gene Name Homo sapiens protein kinase D1 (PRKD1), transcript variant 2
Background PKC mu is a novel member of the subgroup of atypical protein kinase Cs (PKC). Deduced protein sequence shows strong homology to conserved domains of members of the PKC subfamily. In vitro phorbol ester binding studies and kinase assays with lysates of cells overexpressing PKC mu showed phorbol ester-independent kinase activity, autophosphorylation, and, in normal rat kidney (NRK) cells, predominant phosphorylation of a 30kD protein at serine residues. Data suggest a role of PKC mu in signal transduction pathways related to growth control. PKC mu is a cytosolic protein, which upon binding to the trans-Golgi network (TGN) regulates the fission of transport carriers specifically destined to the cell surface. Mutation of serines 744/748 to alanines in the activation loop of intact PKD inhibits its localization to the TGN. Moreover, anti-phospho-PKD antibody, which recognizes only the activated form of PKD, recognizes the TGN-bound PKD. Thus, activation of intact PKD is important for binding to the TGN. Results demonstrate that betagamma subunits of the heterotrimeric G proteins directly activates PKD by interacting with its pleckstrin homology domain.
Synonyms Protein kinase D, PKC D1, PKD, PKD1, PRKCM, nPKC-D1, nPKC-mu, PKC mu, Protein kinase C mu type
Reference Data
Protein Families Druggable Genome, Protein Kinase

{0} Product Review(s)

0 Product Review(s) Submit review

Be the first one to submit a review

Product Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.